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Addgene

pET-His-SUMO-LS4
(Plasmid #228450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Total vector size (bp) 6342
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LS4
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGATGCGTCCGGCGTAGAGG
  • 3′ sequencing primer AGGGGTTATGCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-His-SUMO-LS4 was a gift from Keisuke Fukunaga (Addgene plasmid # 228450 ; http://n2t.net/addgene:228450 ; RRID:Addgene_228450)

  • For your References section:

    Structural insights into lab-coevolved RNA-RBP pairs and applications of synthetic riboswitches in cell-free system. Fukunaga K, Teramoto T, Nakashima M, Ohtani T, Katsuki R, Matsuura T, Yokobayashi Y, Kakuta Y. Nucleic Acids Res. 2025 Mar 20;53(6):gkaf212. doi: 10.1093/nar/gkaf212. 10.1093/nar/gkaf212 PubMed 40119732
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