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pCMV-THRA-RFP-EGFP
(Plasmid #228537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    NovoPro
  • Backbone size w/o insert (bp) 4445
  • Total vector size (bp) 12547
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Kanamycin (final 50 ug/ml)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thyroid Hormone Receptor Alpha
  • Alt name
    THRA
  • Alt name
    TRalpha
  • Alt name
    THRalpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    12547
  • GenBank ID
    ENSG00000126351
  • Entrez Gene
    THRA (a.k.a. AR7, CHNG6, EAR7, ERB-T-1, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRalpha, THRalpha1, THRalpha2, TRalpha, TRalpha1, TRalpha2, c-ERBA-1, c-erbA)
  • Promoter CMV
  • Tags / Fusion Proteins
    • tagRFP (C terminal on insert)
    • eGFP (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-THRA-RFP-EGFP was a gift from Markus Schuelke (Addgene plasmid # 228537 ; http://n2t.net/addgene:228537 ; RRID:Addgene_228537)

  • For your References section:

    Bichromatic Splicing Detector Allows Quantification of THRA1 and THRA2 Splicing Isoforms in Single Cells by Fluorescent Live-Cell Imaging. Graceffo E, Pedersen E, Rosario M, Krude H, Schuelke M. Int J Mol Sci. 2024 Dec 17;25(24):13512. doi: 10.3390/ijms252413512. 10.3390/ijms252413512 PubMed 39769274
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