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Addgene

pM-EGFP
(Plasmid #228622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228622 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMulti
  • Backbone manufacturer
    Birtwistle lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter CMV

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.23.619855 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM-EGFP was a gift from Marc Birtwistle (Addgene plasmid # 228622 ; http://n2t.net/addgene:228622 ; RRID:Addgene_228622)

  • For your References section:

    Genetically Encoded Fluorescence Barcodes Allow for Single-Cell Analysis via Spectral Flow Cytometry. Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR. ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 10.1021/acssynbio.4c00807. Epub 2025 May 6. 10.1021/acssynbio.4c00807 PubMed 40326708
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