p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE
(Plasmid
#228766)
-
Purposeinsert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27K
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 228766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonep48
-
Backbone manufacturerFerdinando Pucci
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namep27 kinase inhibitor
-
SpeciesM. musculus (mouse)
-
Entrez GeneCdkn1b (a.k.a. Kip1, p27, p27Kip1)
-
Tag
/ Fusion Protein
- mTagBFP2 (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://ssrn.com/abstract=4912248 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE was a gift from Ferdinando Pucci (Addgene plasmid # 228766 ; http://n2t.net/addgene:228766 ; RRID:Addgene_228766) -
For your References section:
Senescent Cell-Derived Extracellular Vesicles Inhibit Cancer Recurrence by Coordinating Immune Surveillance. Ziglari T, Calistri NL, Finan JM, Derrick DS, Nakayasu ES, Burnet MC, Kyle JE, Hoare M, Heiser LM, Pucci F. Cancer Res. 2025 Mar 3;85(5):859-874. doi: 10.1158/0008-5472.CAN-24-0875. 10.1158/0008-5472.CAN-24-0875 PubMed 39804967