YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G
(Plasmid
#228889)
-
PurposeAAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3G under the control of a hSyn promotor
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepAAV
- Total vector size (bp) 6416
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGFP-GEMINI(A); rtTA3G
-
SpeciesSynthetic
- Promoter TRE; hsyn
-
Tag
/ Fusion Protein
- gfp (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ctaccctcgtaaagg
- (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.07.16.664392 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G was a gift from Dingchang Lin (Addgene plasmid # 228889 ; http://n2t.net/addgene:228889 ; RRID:Addgene_228889) -
For your References section:
Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo. Yan Y, Lu J, Li Z, Zhao Z, Shay TF, Wang S, Lei Y, Wang Y, Chen W, Parker P, Yang H, Qi A, Sun Y, Bergles DE, Baker D, Lin D. bioRxiv 2025.07.16.664392 10.1101/2025.07.16.664392