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pET28a-RANGAP1-CTD
(Plasmid #228947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone size w/o insert (bp) 5237
  • Total vector size (bp) 5812
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RANGAP1-CTD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    575
  • Mutation
    contains aa 418-587 only
  • GenBank ID
    CR456557.1
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)
  • Promoter T7-lac
  • Tag / Fusion Protein
    • His tag and thrombine cleavage site : MGSSHHHHHHSSGLVPRGSHM (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Optimized sequences supplied by Genecust

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-RANGAP1-CTD was a gift from Marcin Suskiewicz (Addgene plasmid # 228947 ; http://n2t.net/addgene:228947 ; RRID:Addgene_228947)
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