pET28a-shortSUMO1
(Plasmid #228951)

• Purpose: express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized
• Depositing Lab: Marcin Suskiewicz
• Publication: Production and purification of SUMO-UBC9 and SUMO-RANGAP1CTD (unpublished)

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5296
• Total vector size (bp): 5587
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUMO1
• Species: H. sapiens (human)
• Insert Size (bp): 291
• Mutation: deleted amino acids 1-17
• GenBank ID: U38784.1
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Promoter: T7-lac
• Tag / Fusion Protein:
  • His tag and thrombine cleavage site : MGSSHHHHHHSSGLVPRGSHM (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry