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pRK5-HA-Ubiquitin-K6
(Plasmid #22900)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22900 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin C
  • Alt name
    Ub
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    220
  • Mutation
    K6 only, all other lysines mutated to arginines
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pRK5-HA-Ubiquitin-K6 was derived from pRK5-HA-Ubiquitin-K0 originally generated by the Dr. Ted Dawson laboratory (Lim KL et al. J Neuroscience. 2005. Feb 23. 25(8): 2002-9).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-Ubiquitin-K6 was a gift from Sandra Weller (Addgene plasmid # 22900 ; http://n2t.net/addgene:22900 ; RRID:Addgene_22900)
  • For your References section:

    Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear protein quality control centers during HSV-1 infection. Livingston CM, Ifrim MF, Cowan AE, Weller SK. PLoS Pathog. 2009 Oct . 5(10):e1000619. 10.1371/journal.ppat.1000619 PubMed 19816571