Skip to main content
Addgene

Lenti-U6-sgRNA-BFP-Puro
(Plasmid #229014)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229014 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    CROPseq-Guide-Puro
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.21.633969 for the bioRxiv preprint and find the full Perturb-seq protocol on Protocols.io: https://www.protocols.io/view/experimental-procedures-for-tf-perturb-seq-ut-sout-14egn635pl5d/v1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-U6-sgRNA-BFP-Puro was a gift from Gary Hon (Addgene plasmid # 229014 ; http://n2t.net/addgene:229014 ; RRID:Addgene_229014)

  • For your References section:

    Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells. Sivakumar S, Wang Y, Goetsch SC, Pandit V, Wang L, Zhao H, Sundarrajan A, Armendariz D, Takeuchi C, Nzima M, Chen WC, Dederich AE, El Hayek L, Gao T, Ghazawi R, Gogate A, Kaur K, Kim HB, McCoy MK, Niederstrasser H, Oura S, Pinzon-Arteaga CA, Sanghvi M, Schmitz DA, Yu L, Zhang Y, Zhou Q, Kraus WL, Xu L, Wu J, Posner BA, Chahrour MH, Hon GC, Munshi NV. bioRxiv [Preprint]. 2025 Jan 23:2025.01.21.633969. doi: 10.1101/2025.01.21.633969. 10.1101/2025.01.21.633969 PubMed 39896670
Related items: