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Addgene

PiggyBac-APEX-V5-NES
(Plasmid #229082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229082 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    K4-PB-TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NES
  • Alt name
    nuclear export signal
  • Alt name
    TDP43
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Promoter TRE3G
  • Tags / Fusion Proteins
    • V5 (N terminal on insert)
    • APEX (D11G mutation) (N terminal on insert)
    • BFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamhI (unknown if destroyed)
  • 3′ cloning site NruI (unknown if destroyed)
  • 5′ sequencing primer cccttcggatgtggcttga
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned insert in the place of NGN2 in the K4-PB-TO_NGN2 plasmid received from Michael Ward

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac-APEX-V5-NES was a gift from Eran Hornstein (Addgene plasmid # 229082 ; http://n2t.net/addgene:229082 ; RRID:Addgene_229082)
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