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pMT-FLAG-DmAgo1
(Plasmid #229236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229236 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMT
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Argonaute1
  • Alt name
    Ago1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2850
  • Entrez Gene
    AGO1 (a.k.a. Dmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago-1, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121)
  • Promoter MT promoter
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.16.623948 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-FLAG-DmAgo1 was a gift from Karina Jouravleva (Addgene plasmid # 229236 ; http://n2t.net/addgene:229236 ; RRID:Addgene_229236)

  • For your References section:

    Biochemical principles of miRNA targeting in flies. Vega-Badillo J, Zamore PD, Jouravleva K. Nat Commun. 2026 Jan 20. doi: 10.1038/s41467-026-68360-0. 10.1038/s41467-026-68360-0 PubMed 41559052
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