pRSF_AaRbcLS
(Plasmid #229505)

• Purpose: Expresses Anthoceros agrestis Rubisco large and small subunit (C terminus hexahistidine)
• Depositing Lab: Laura Gunn
• Publication: Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2.

Backbone

• Vector backbone: pRSFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3829
• Total vector size (bp): 5667
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Rubisco large subunit
• Alt name: RbcL
• Species: Anthoceros agrestis
• Insert Size (bp): 1426
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Other
• 5′ sequencing primer: Not available

Gene/Insert 2

• Gene/Insert name: Rubisco small subunit
• Alt name: RbcS
• Species: Anthoceros agrestis
• Insert Size (bp): 378
• Mutation: N terminus chloroplast transit peptide truncation
• Promoter: T7
• Tag / Fusion Protein:
  • hexahistidine (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Other
• 5′ sequencing primer: Not available

Resource Information

• Supplemental Documents:
  • pRSF_AaRbcLS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RbcL and RbcS fragments were ordered from Twist Bioscience, construct was formed with infusion cloning to an amplifed pRSF backbone. Design of Anthoceros agrestis Rubisco expression plasmid mirrors Arabidopsis thaliana expression casette of pAtRbcLS as shown in Aigner et al 2017 (10.1126/science.aap9221).