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p11_AaC60αβ/C20
(Plasmid #229506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5677
  • Total vector size (bp) 9733
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Chaperonin 60α
  • Alt name
    Cpn60α
  • Species
    Anthoceros agrestis
  • Mutation
    N terminus chloroplast transit peptide truncation
  • Promoter T7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Chaperonin 60β
  • Alt name
    Cpn60β
  • Species
    Anthoceros agrestis
  • Mutation
    N terminus chloroplast transit peptide truncation
  • Promoter T7

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Chaperonin 20
  • Alt name
    Cpn20
  • Species
    Anthoceros agrestis
  • Mutation
    N terminus chloroplast transit peptide truncation
  • Promoter T7

Cloning Information for Gene/Insert 3

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Synthesis from Genscript. Design of the placement of Anthoceros agrestis Chaperonin 60αβ/Chaperonin 20 inserts was mirroring pAtC60αβ/C20 as shown in Aigner et al 2017 (10.1126/science.aap9221).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p11_AaC60αβ/C20 was a gift from Laura Gunn (Addgene plasmid # 229506 ; http://n2t.net/addgene:229506 ; RRID:Addgene_229506)

  • For your References section:

    Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems. Oh ZG, Robison TA, Loh DH, Ang WSL, Ng JZY, Li FW, Gunn LH. Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2. 10.1016/j.molp.2024.10.013 PubMed 39491367
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