pCDF_AaRaf1/Raf2/RbcX2/BSD2
(Plasmid #229507)

• Purpose: Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2
• Depositing Lab: Laura Gunn
• Publication: Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2.

Backbone

• Vector backbone: pCDFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3781
• Total vector size (bp): 6333
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Rubisco accumulation factor 1
• Alt name: Raf1
• Species: Anthoceros agrestis
• Insert Size (bp): 1176
• Mutation: N terminus chloroplast transit peptide truncation
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Golden Gate
• 5′ sequencing primer: Not available

Gene/Insert 2

• Gene/Insert name: Rubisco accumulation factor 2
• Alt name: Raf2
• Species: Anthoceros agrestis
• Insert Size (bp): 513
• Mutation: N terminus chloroplast transit peptide truncation
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Golden Gate
• 5′ sequencing primer: Not available

Gene/Insert 3

• Gene/Insert name: RbcX2
• Species: Anthoceros agrestis
• Insert Size (bp): 447
• Mutation: N terminus chloroplast transit peptide truncation
• Promoter: T7

Cloning Information for Gene/Insert 3

• Cloning method: Golden Gate
• 5′ sequencing primer: Not available

Gene/Insert 4

• Gene/Insert name: BSD2
• Alt name: BUNDLE SHEATH DEFECTIVE 2
• Species: Anthoceros agrestis
• Insert Size (bp): 267
• Mutation: N terminus chloroplast transit peptide truncation
• GenBank ID:
• Promoter: T7

Cloning Information for Gene/Insert 4

• Cloning method: Golden Gate
• 5′ sequencing primer: Not Available

Resource Information

• Supplemental Documents:
  • RRXB [RbcXII according to AtRbcXII], tubes labelled as AaRRXB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.