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pCDF_AtRaf1/Raf2/BSD2
(Plasmid #229511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 6311
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Rubisco accumulation factor 1
  • Alt name
    Raf1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1176
  • Mutation
    N terminus chloroplast transit peptide truncation
  • Entrez Gene
    AT3G04550 (a.k.a. AT3G04550, F7O18.2, F7O18_2)
  • Promoter T7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Rubisco accumulation factor 2
  • Alt name
    Raf2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    516
  • Mutation
    N terminus chloroplast transit peptide truncation
  • Entrez Gene
    AT5G51110 (a.k.a. AT5G51110, MWD22.5, MWD22_5)
  • Promoter T7

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    BSD2
  • Alt name
    BUNDLE SHEATH DEFECTIVE 2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    249
  • Mutation
    N terminus chloroplast transit peptide truncation
  • GenBank ID
    AEE78314.1
  • Promoter T7

Cloning Information for Gene/Insert 3

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Synthesis from Genscript. Design of the three Rubisco assembly factor inserts was mirroring pAtR1/R2/RX/B2 as shown in Aigner et al 2017 (10.1126/science.aap9221).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF_AtRaf1/Raf2/BSD2 was a gift from Laura Gunn (Addgene plasmid # 229511 ; http://n2t.net/addgene:229511 ; RRID:Addgene_229511)

  • For your References section:

    Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems. Oh ZG, Robison TA, Loh DH, Ang WSL, Ng JZY, Li FW, Gunn LH. Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2. 10.1016/j.molp.2024.10.013 PubMed 39491367
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