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pRSF_AaNHis_RbcL
(Plasmid #229517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 5286
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rubisco large subunit
  • Alt name
    RbcL
  • Species
    S. pombe (fission yeast); Anthoceros agrestis
  • Insert Size (bp)
    1446
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Synthesis from Genscript with N terminus histidine tag was added to N-terminus of Rubisco large subunit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF_AaNHis_RbcL was a gift from Laura Gunn (Addgene plasmid # 229517 ; http://n2t.net/addgene:229517 ; RRID:Addgene_229517)

  • For your References section:

    Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems. Oh ZG, Robison TA, Loh DH, Ang WSL, Ng JZY, Li FW, Gunn LH. Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2. 10.1016/j.molp.2024.10.013 PubMed 39491367
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