pRSF_PcRbcLS
(Plasmid #229519)

• Purpose: Expresses Phaeoceros carolinianus Rubisco large and small subunit (C terminus hexahistidine)
• Depositing Lab: Laura Gunn
• Publication: Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2.

Backbone

• Vector backbone: pRSFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3829
• Total vector size (bp): 5523
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Rubisco large subunit
• Alt name: RbcL
• Species: Phaeoceros carolinianus
• Insert Size (bp): 1428
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Not available

Gene/Insert 2

• Gene/Insert name: Rubisco small subunit
• Alt name: RbcS
• Species: Phaeoceros carolinianus
• Insert Size (bp): 381
• Mutation: N terminus chloroplast transit peptide truncation
• Promoter: T7
• Tag / Fusion Protein:
  • hexahistidine (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Not available

Resource Information

• Supplemental Documents:
  • 4_Phaeoceros_carolinanus_pRSFDuet-1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Design of Phaeoceros carolinianus Rubisco expression plasmid mirrors Anthoceros agrestis Rubisco expression plasmid, pAaRbcLS as shown in Oh et al 2024 (10.1016/j.molp.2024.10.013).