pcDNA3-p53-G609A
(Plasmid
#229532)
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PurposeExpresses p53 mutant G609A in mamallian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep53-CDS
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SpeciesH. sapiens (human)
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Insert Size (bp)1182
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Mutationc.G609A
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-p53-G609A was a gift from Robin Fåhraeus (Addgene plasmid # 229532 ; http://n2t.net/addgene:229532 ; RRID:Addgene_229532) -
For your References section:
A cancer-associated TP53 synonymous mutation induces synthesis of the p53 isoform p53/47. Sajwan R, Wang L, Casar-Borota O, Karakostis K, Chen S, Fahraeus R, Gu X, Vadivel Gnanasundram S. Br J Cancer. 2025 Jul 26. doi: 10.1038/s41416-025-03127-w. 10.1038/s41416-025-03127-w PubMed 40715694