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Addgene

TB204 △hisD proB74
(Bacterial strain #229550)

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Full plasmid sequence is not available for this item.

No maps are available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 229550 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Vector type
    This is a strain, not a plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TB204 △hisD proB74
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MG1655 attP21::PR-sfGFP::frt hisD:frt proBA::proB74proA
  • Species
    n/a
  • Mutation
    in proB: Aspartic acid 107 mutated to asparagine (D107N) GAT->AAT

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Primers for sequence verification of proB mutation:
Fwd - prEP169, cgcggttatgtgaagaacgt
Rev - prEP170, gtgatgtcgcgttataccgg

Please visit https://doi.org/10.1101/2024.07.19.604250 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB204 △hisD proB74 was a gift from Yolanda Schaerli (Addgene plasmid # 229550)

  • For your References section:

    Uptake and leakage rates differentially shape community arrangement and composition of microbial consortia. Pignon E, Holló G, Steiner T, van Vliet S, Schaerli Y. ISME. 2025 (in press)
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