pAAV.RMacL3-12.EGFP.BCC3.WPRE
(Plasmid
#229627)
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PurposeL3 pyramidal neuron Enhancer to drive transgene expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEMS2115 (Addgene Plasmid #49140)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRMacL3-12.EGFP.BCC3
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.01.626253 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.RMacL3-12.EGFP.BCC3.WPRE was a gift from William Stauffer (Addgene plasmid # 229627 ; http://n2t.net/addgene:229627 ; RRID:Addgene_229627) -
For your References section:
Machine learning identification of enhancers in the rhesus macaque genome. He J, Phan BN, Kerkhoff WG, Alikaya A, Hong T, Brull OR, Fredericks JM, Sedorovitz M, Srinivasan C, Leone MJ, Wirfel OM, Brown A, Dauby S, Tittle RK, Lin MK, Hooks BM, Bostan AC, Gharbawie OA, Byrne LC, Pfenning AR, Stauffer WR. Neuron 2025. 113, 10. doi: 10.1016/j.neuron.2025.04.030 10.1016/j.neuron.2025.04.030