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pGWB515_35S-HA-(ΔTP)DM3(Col-0) Q345R
(Plasmid #229720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229720 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGWB515
  • Backbone manufacturer
    Tsuyoshi Nakagawa (Addgene plasmid #74857)
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DM3(Col-0)
  • Alt name
    At3g61540
  • Species
    A. thaliana (mustard weed)
  • Mutation
    amino acid 1-52 were deleted, Q345R
  • Entrez Gene
    AT3G61540 (a.k.a. AT3G61540)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.28.620575 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWB515_35S-HA-(ΔTP)DM3(Col-0) Q345R was a gift from Eunyoung Chae (Addgene plasmid # 229720 ; http://n2t.net/addgene:229720 ; RRID:Addgene_229720)

  • For your References section:

    Structural determinants of DANGEROUS MIX 3, an alpha/beta hydrolase that triggers NLR-mediated genetic incompatibility in plants. Wan WL, Kim G, Kim N, Tan YY, Watari M, Charoennit N, Lee RRQ, Phang IRK, Wang J, Liew YY, Hu D, Ng SK, Zhang Y, Jang IC, Song JJ, Chae E.. Molecular Cell, Volume 85, Issue 14 10.1016/j.molcel.2025.06.021
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