pGWB512_35S-FLAG-DM3(Col-0)68-510
(Plasmid
#229733)
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PurposeStable transformation or transient expression of gene of interest in plants
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGWB512
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Backbone manufacturerTsuyoshi Nakagawa (Addgene plasmid #74854)
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDM3(Col-0)
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Alt nameAt3g61540
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SpeciesA. thaliana (mustard weed)
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Mutationamino acid 1-67 were deleted, 511-515 were deleted
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Entrez GeneAT3G61540 (a.k.a. AT3G61540)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.10.28.620575 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB512_35S-FLAG-DM3(Col-0)68-510 was a gift from Eunyoung Chae (Addgene plasmid # 229733 ; http://n2t.net/addgene:229733 ; RRID:Addgene_229733) -
For your References section:
Structural determinants of DANGEROUS MIX 3, an alpha/beta hydrolase that triggers NLR-mediated genetic incompatibility in plants. Wan WL, Kim G, Kim N, Tan YY, Watari M, Charoennit N, Lee RRQ, Phang IRK, Wang J, Liew YY, Hu D, Ng SK, Zhang Y, Jang IC, Song JJ, Chae E.. Molecular Cell, Volume 85, Issue 14 10.1016/j.molcel.2025.06.021