AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)
(Plasmid #229775)

• Purpose: enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer
• Depositing Labs: The Allen Institute for Brain Science, Jonathan Ting
• Publication: Matveev et al bioRxiv 2024.11.01.621418

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ChrimsonR-tdTomato
• Species: Synthetic
• Insert Size (bp): 2502
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AiP12068.gb
• A portion of this plasmid was derived from a plasmid made by: Edward Boyden (Addgene plasmid # 59171); the ChrimsonR-tdTomato was subcloned from it.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.01.621418 for bioRxiv preprint.