pCAGEN-Dre-nls (JDW 1131)
(Plasmid
#229809)
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PurposeA CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGEN-DEST
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDRE recombinase-nls
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SpeciesSynthetic
- Promoter CAG
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Tag
/ Fusion Protein
- SV40 NLS (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGEN-Dre-nls (JDW 1131) was a gift from Joshua Wythe (Addgene plasmid # 229809 ; http://n2t.net/addgene:229809 ; RRID:Addgene_229809) -
For your References section:
Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems. Gillespie W, Zhang Y, Ruiz OE, Cerda J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien CB, Kwan KM, Arenkiel BR, Devine WP, Wythe JD. bioRxiv [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 10.1101/2024.07.13.603267. 10.1101/2024.07.13.603267 PubMed 39026881