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p5E-Crestin-pro (JDW 1320)
(Plasmid #229830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229830 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDONR P4-P1r
  • Vector type
    Gateway entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Crestin promoter, c-fos minimal promoter, and b-globin intron
  • Species
    D. rerio (zebrafish)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-Crestin-pro (JDW 1320) was a gift from Joshua Wythe (Addgene plasmid # 229830 ; http://n2t.net/addgene:229830 ; RRID:Addgene_229830)

  • For your References section:

    MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems. Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, Kwan KM, Arenkiel BR, Devine WP, Wythe JD. Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308. Epub 2025 Nov 13. 10.1242/dev.204308 PubMed 41230856
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