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Addgene

pAAV-puro_TRE3VG_EGFP-Responder
(Plasmid #230050)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230050 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-Puro_TRE-EGFP
  • Backbone size w/o insert (bp) 5571
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRE3VG_EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    1082
  • Promoter GAAGACAATAGCAGGCATG

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GAAGACAATAGCAGGCATG
  • 3′ sequencing primer GGTTCAGGGGGAGGTGTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-puro_TRE3VG_EGFP-Responder was a gift from Alessandro Bertero (Addgene plasmid # 230050 ; http://n2t.net/addgene:230050 ; RRID:Addgene_230050)

  • For your References section:

    Overcoming the Silencing of Doxycycline-Inducible Promoters in hiPSC-derived Cardiomyocytes. Guichardaz M, Bottini S, Balmas E, Bertero A. Open Res Eur. 2024 Dec 18;4:266. doi: 10.12688/openreseurope.19024.1. eCollection 2024. 10.12688/openreseurope.19024.1 PubMed 39926351
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