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pCLYBL-Neo_TRE3VG_mCherry_Responder
(Plasmid #230054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230054 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHEC015_CLYBL_neo_CAG_dCas9-P2A-T2A-mCherry
  • Backbone size w/o insert (bp) 7887
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRE3VG_mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1351
  • Promoter TRE3VG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tccaggacggagtcagtgag
  • 3′ sequencing primer GAATTCGAGCTCGGTACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLYBL-Neo_TRE3VG_mCherry_Responder was a gift from Alessandro Bertero (Addgene plasmid # 230054 ; http://n2t.net/addgene:230054 ; RRID:Addgene_230054)

  • For your References section:

    Overcoming the Silencing of Doxycycline-Inducible Promoters in hiPSC-derived Cardiomyocytes. Guichardaz M, Bottini S, Balmas E, Bertero A. Open Res Eur. 2024 Dec 18;4:266. doi: 10.12688/openreseurope.19024.1. eCollection 2024. 10.12688/openreseurope.19024.1 PubMed 39926351
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