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Addgene

pKDcas9 57 cerulean
(Plasmid #230959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 230959 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pdCas9
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • gRNA/shRNA sequence
    see annotated Genbank file
  • Species
    Other
  • Mutation
    additional tetO

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKDcas9 57 cerulean was a gift from Andrew Griffiths (Addgene plasmid # 230959 ; http://n2t.net/addgene:230959 ; RRID:Addgene_230959)

  • For your References section:

    Global regulators enable bacterial adaptation to a phenotypic trade-off. Deyell M, Opuu V, Griffiths AD, Tans SJ, Nghe P. iScience. 2024 Dec 9;28(1):111521. doi: 10.1016/j.isci.2024.111521. eCollection 2025 Jan 17. 10.1016/j.isci.2024.111521 PubMed 39811663
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