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pSicoFlipped-CAG-GFP-WPRE-bGHpA
(Plasmid #231018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231018 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSico/Pooled Library #186334
  • Backbone manufacturer
    Tyler Jacks
  • Backbone size w/o insert (bp) 7595
  • Total vector size (bp) 8415
  • Modifications to backbone
    The transgene is flipped relative to LTRs, and requires co-transfection with Nodamura B2 (Addgene #17228) during viral packaging to obtain high titers.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced GFP
  • Alt name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TCGTAACTAGCGCTACCG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSicoFlipped-CAG-GFP-WPRE-bGHpA was a gift from Tomasz Nowakowski (Addgene plasmid # 231018 ; http://n2t.net/addgene:231018 ; RRID:Addgene_231018)

  • For your References section:

    Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders. Shin D, Kim CN, Ross J, Hennick KM, Wu SR, Paranjape N, Leonard R, Wang JC, Keefe MG, Pavlovic BJ, Donohue KC, Moreau C, Wigdor EM, Larson HH, Allen DE, Cadwell CR, Bhaduri A, Popova G, Bearden CE, Pollen AA, Jacquemont S, Sanders SJ, Haussler D, Wiita AP, Frost NA, Sohal VS, Nowakowski TJ. Cell Stem Cell. 2024 Mar 7;31(3):421-432.e8. doi: 10.1016/j.stem.2024.01.010. Epub 2024 Feb 20. 10.1016/j.stem.2024.01.010 PubMed 38382530
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