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pBK-EcTrpRS(H14)
(Plasmid #231129)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231129 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBK
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E. coli tryptophanyl tRNA synthetase
  • Alt name
    EcWRS
  • Species
    E. coli
  • Mutation
    S8A, V144G, V146C

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBK-EcTrpRS(H14) was a gift from Abhishek Chatterjee (Addgene plasmid # 231129 ; http://n2t.net/addgene:231129 ; RRID:Addgene_231129)

  • For your References section:

    Cell-selective multiplexed bioorthogonal noncanonical amino acid tagging for nascent proteomics. Loynd C, Singha Roy SJ, Canarelli SE, Bak DW, Sundaresh B, Babbitz Z, van Opijnen T, Weerapana E, Chatterjee A. Nat Chem Biol. 2025 Oct 2. doi: 10.1038/s41589-025-02039-3. 10.1038/s41589-025-02039-3 PubMed 41039161
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