pSGAb/pEvol-AbTrpRS(H14)
(Plasmid
#231133)
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PurposeExpresses mutant A. baumannii TrpRS from an inducible lac promoter for proteome-wide incorporation of tryptophan analogues.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 231133 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSGAb
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Vector typeBacterial Expression ; Acinetobacter baumannii expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA. baumannii tryptophanyl tRNA synthetase
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Alt nameAbWRS
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SpeciesE. coli
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MutationT12A, V151G, V153C
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSGAb/pEvol-AbTrpRS(H14) was a gift from Abhishek Chatterjee (Addgene plasmid # 231133 ; http://n2t.net/addgene:231133 ; RRID:Addgene_231133) -
For your References section:
Cell-selective multiplexed bioorthogonal noncanonical amino acid tagging for nascent proteomics. Loynd C, Singha Roy SJ, Canarelli SE, Bak DW, Sundaresh B, Babbitz Z, van Opijnen T, Weerapana E, Chatterjee A. Nat Chem Biol. 2025 Oct 2. doi: 10.1038/s41589-025-02039-3. 10.1038/s41589-025-02039-3 PubMed 41039161