pAVEC1_N-mEGFP
(Plasmid
#231271)
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Purpose(Empty Backbone) For stably expressing fusion proteins with N terminally tagged mEGFP in plants
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 231271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneBackbone assembled from fragments of pENTR, pEG103, and NPTII
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Vector typePlant Expression
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Selectable markersBasta
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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MutationN/A
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
No gene inserted in primary locus, this is a vector backbone. pAVEC1 has a bialaphos resistance (BAR) cassette inserted in a secondary locus for glufosinate selection during plant transformation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAVEC1_N-mEGFP was a gift from Jacob Brunkard (Addgene plasmid # 231271 ; http://n2t.net/addgene:231271 ; RRID:Addgene_231271) -
For your References section:
Seamless, modular binary vectors for plant cell and molecular biology. Greenhalgh R, Horner W, Klimpel KA, Chatterjee S, Scarpin MR, Brunkard JO. Plant Biotechnol J. 2025 Jun 26. doi: 10.1111/pbi.70233. 10.1111/pbi.70233 PubMed 40574375
