pAVEC2_N-TurboID
(Plasmid
#231293)
-
Purpose(Empty Backbone) For transiently expressing fusion proteins with N terminal TurboID for proximity labeling in plants (includes p19 silencing suppressor)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 231293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backboneBackbone assembled from fragments of pENTR, pEG103, and NPTII
-
Vector typePlant Expression
-
Tag
/ Fusion Protein
- TurboID (N terminal on insert)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameNone
-
MutationN/A
-
Tag
/ Fusion Protein
- TurboID (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
No gene inserted in primary locus, this is a vector backbone. pAVEC2 has a p19 expression cassette (TBSV p19 silencing supressor from tomato bushy stunt virus) inserted into a secondary locus for increased protein expression in plant transient expression experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAVEC2_N-TurboID was a gift from Jacob Brunkard (Addgene plasmid # 231293) -
For your References section:
Seamless, modular binary vectors for plant cell and molecular biology. Greenhalgh R, Horner W, Klimpel KA, Chatterjee S, Scarpin MR, Brunkard JO. Plant Biotechnol J. 2025 Jun 26. doi: 10.1111/pbi.70233. 10.1111/pbi.70233 PubMed 40574375