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pAAV-GFAP-spCas9
(Plasmid #231403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231403 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFAP promoter
  • Species
    M. musculus (mouse)
  • Mutation
    Changed RSV promoter to hSYN promoter
  • Entrez Gene
    RIC8B (a.k.a. RIC8, hSyn)
  • Promoter GFAP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (unknown if destroyed)
  • 3′ cloning site Age1 (not destroyed)
  • 5′ sequencing primer ggcctctagatctgcaagca
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Replaced RSV promoter from Plasmid #87916 Lei lab with GFAP promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-GFAP-spCas9 was a gift from Arjen Boender & Larry Young (Addgene plasmid # 231403 ; http://n2t.net/addgene:231403 ; RRID:Addgene_231403)
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