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pBIG1a-PAF1CdeltaCDC73
(Plasmid #231746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIG1a
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CTR9
  • Species
    H. sapiens (human)
  • Entrez Gene
    CTR9 (a.k.a. SH2BP1, TSBP, p150, p150TSP)
  • Tag / Fusion Protein
    • 3C-Strep(II) (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    PAF1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PAF1 (a.k.a. F23149_1, PD2)

Gene/Insert 3

  • Gene/Insert name
    LEO1
  • Species
    H. sapiens (human)
  • Entrez Gene
    LEO1 (a.k.a. RDL)

Gene/Insert 4

  • Gene/Insert name
    WDR61
  • Species
    H. sapiens (human)
  • Entrez Gene
    SKIC8 (a.k.a. REC14, SKI8, WDR61)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.14.618141 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBIG1a-PAF1CdeltaCDC73 was a gift from Jesper Svejstrup (Addgene plasmid # 231746 ; http://n2t.net/addgene:231746 ; RRID:Addgene_231746)

  • For your References section:

    PAF1C-mediated activation of CDK12/13 kinase activity is critical for CTD phosphorylation and transcript elongation. Martinez DL, Todorovski I, Gonzalez MN, Rusimbi C, Blears D, Khallou N, Han Z, Dirac-Svejstrup B, Svejstrup JQ. Molecular Cell. 01 Mar 2025. doi: 10.1016/j.molcel.2025.04.012 10.1016/j.molcel.2025.04.012
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