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pGEM-7zf(+)_mmSRA1
(Plasmid #232032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232032 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEM-7Zf(+)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2997
  • Total vector size (bp) 3618
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sra1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    649
  • Entrez Gene
    Sra1 (a.k.a. Sra, Srap, Straa1, Strra1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-7zf(+)_mmSRA1 was a gift from Primo Schaer (Addgene plasmid # 232032 ; http://n2t.net/addgene:232032 ; RRID:Addgene_232032)

  • For your References section:

    The TDG protein environment connects active DNA demethylation with chromatin and RNA biology. Richina F, Noreen F, Bauer C, Weber A, Kunz C, Buczak K, Schwarz SD, Wu F, Schurmann D, Schar P. Cell Mol Life Sci. 2025 Nov 25. doi: 10.1007/s00018-025-05943-y. 10.1007/s00018-025-05943-y PubMed 41291101
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