PC2328
(Plasmid
#232089)
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PurposeMammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 5974
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameduAb with cloning Esp3I restriction site
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SpeciesH. sapiens (human)
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Insert Size (bp)615
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-Forward
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-4670386/v1 for Research Square preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PC2328 was a gift from Pranam Chatterjee (Addgene plasmid # 232089 ; http://n2t.net/addgene:232089 ; RRID:Addgene_232089) -
For your References section:
Programmable Protein Stabilization with Language Model-Derived Peptide Guides. Hong L, Ye T, Wang TZ, Srijay D, Zhao L, Watson R, Vincoff S, Chen T, Kholina K, Goel S, DeLisa MP, Chatterjee P. Nat Commun 16, 3555 (2025). https://doi.org/10.1038/s41467-025-58872-6 10.21203/rs.3.rs-4670386/v1 PubMed 39108486