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Addgene

pLV EF1alpha INTS4 IRES Cherry
(Plasmid #232109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232109 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLV EF1alpha MCS IRES Cherry
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    INTS4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2886
  • Mutation
    Wild Type
  • Entrez Gene
    INTS4 (a.k.a. INT4, MST093)
  • Promoter EF 1alpha
  • Tag / Fusion Protein
    • IRES CHERRY (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ATGGCGGCGCACCTTAAGAAGCGGGTTTATGAGGAATTCACG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.06.08.597999 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV EF1alpha INTS4 IRES Cherry was a gift from Tom Misteli (Addgene plasmid # 232109 ; http://n2t.net/addgene:232109 ; RRID:Addgene_232109)

  • For your References section:

    Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens. Sood V, Holewinski R, Andresson T, Larson DR, Misteli T. Mol Cell. 2025 Mar 6;85(5):913-928.e8. doi: 10.1016/j.molcel.2025.01.022. Epub 2025 Feb 19. 10.1016/j.molcel.2025.01.022 PubMed 39978338
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