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LpABCB1-NmCherry
(Plasmid #232276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232276 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCS2+8
  • Backbone size w/o insert (bp) 4126
  • Total vector size (bp) 8809
  • Modifications to backbone
    None
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABCB1
  • Alt name
    Pgp; P-glycoprotein
  • Species
    Lytechinus pictus
  • Insert Size (bp)
    4683
  • GenBank ID
  • Promoter SP6
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LpABCB1-NmCherry was a gift from Amro Hamdoun (Addgene plasmid # 232276 ; http://n2t.net/addgene:232276 ; RRID:Addgene_232276)

  • For your References section:

    Generation of a homozygous mutant drug transporter (ABCB1) knockout line in the sea urchin Lytechinus pictus. Vyas H, Schrankel CS, Espinoza JA, Mitchell KL, Nesbit KT, Jackson E, Chang N, Lee Y, Warner J, Reitzel A, Lyons DC, Hamdoun A. Development. 2022 Jun 1;149(11):dev200644. doi: 10.1242/dev.200644. Epub 2022 Jun 6. 10.1242/dev.200644 PubMed 35666622
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