mAID(KR)–2×GFP NB(WT)
(Plasmid
#232304)
-
PurposeKR GFP adaptor for C. elegans
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 232304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript
-
Vector typeWorm Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert nameKR GFP adaptor
-
SpeciesSynthetic
Gene/Insert 2
-
Gene/Insert nameOsTIR1(F74G)
-
SpeciesSynthetic
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mAID(KR)–2×GFP NB(WT) was a gift from Masato Kanemaki (Addgene plasmid # 232304 ; http://n2t.net/addgene:232304 ; RRID:Addgene_232304) -
For your References section:
A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins. Islam M, Negishi T, Kitamoto N, Hatoyama Y, Gamo K, Hayashi KI, Kanemaki MT. J Cell Sci. 2025 Jun 9:jcs.263961. doi: 10.1242/jcs.263961. 10.1242/jcs.263961 PubMed 40485297