mCherry-DRP1 K38A
(Plasmid
#232350)
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PurposeExpresses K38A variant of human DRP1 with mCherry tag
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 232350-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 232350-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemCh-alpha tubulin (Addgene Plasmid #49149)
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Backbone manufacturerGia Voeltz Lab
- Total vector size (bp) 6783
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDRP1
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SpeciesH. sapiens (human)
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MutationK38A
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Entrez GeneDNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Other
- 5′ sequencing primer mCherry-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJonathon Friedman, Gia Voeltz Lab: mCh-Drp1 (Addgene Plasmid #49152)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.05.604215 for bioRxiv preprint.
DNA (Catalog # 232350-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 232350-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-DRP1 K38A was a gift from Samantha Lewis (Addgene plasmid # 232350 ; http://n2t.net/addgene:232350 ; RRID:Addgene_232350) -
For your References section:
Spatial analysis of mitochondrial gene expression reveals dynamic translation hubs and remodeling in stress. Begeman A, Smolka JA, Shami A, Waingankar TP, Lewis SC. Sci Adv. 2025 Apr 18;11(16):eads6830. doi: 10.1126/sciadv.ads6830. Epub 2025 Apr 18. 10.1126/sciadv.ads6830 PubMed 40249810
