MKC131_CCR5(SFFV-synEPOR-2A-YFP)
(Plasmid
#232412)
-
PurposeAAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backboneKRD1
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameTruncated Erythropoietin Receptor
-
Alt nametEPOR
-
SpeciesH. sapiens (human)
-
Entrez GeneEPOR (a.k.a. EPO-R)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.04.12.589260 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MKC131_CCR5(SFFV-synEPOR-2A-YFP) was a gift from Kyle Cromer (Addgene plasmid # 232412 ; http://n2t.net/addgene:232412 ; RRID:Addgene_232412) -
For your References section:
Engineering synthetic signaling receptors to enable erythropoietin-free erythropoiesis. Shah AP, Majeti KR, Ekman FK, Selvaraj S, Sharma D, Sinha R, Soupene E, Chati P, Luna SE, Charlesworth CT, McCreary T, Lesch BJ, Tran T, Chu SN, Porteus MH, Kyle Cromer M. Nat Commun. 2025 Jan 29;16(1):1140. doi: 10.1038/s41467-025-56239-5. 10.1038/s41467-025-56239-5 PubMed 39880867