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MB 45t3 thsS(t3)R-sfGFP_mCherry
(Plasmid #232470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232470 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD33
  • Backbone manufacturer
    Beckwith Lab
  • Backbone size w/o insert (bp) 5352
  • Total vector size (bp) 10001
  • Modifications to backbone
    point mutations introduced during cloning
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    thsS(t3)
  • Alt name
    Thiosulfate sensor kinase thsS (Shal_3128) with mutations to enhance performance at 37°C
  • Species
    Shewanella halifaxensis HAW‐EB4
  • Insert Size (bp)
    1785
  • Mutation
    Contains the following mutations K286Q, Q350H, I553V, and F565I, as well as silent point mutations in H51 and S338
  • GenBank ID
    NC_010334
  • Promoter J23104 (ttgacagctagctcagtcctaggtattgtgctagc)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    thsR
  • Alt name
    Thiosulfate response regulator thsR (Shal_3129)
  • Species
    Shewanella halifaxensis HAW‐EB4
  • Insert Size (bp)
    612
  • GenBank ID
    NC_010334
  • Promoter J23105 (TTTACGGCTAGCTCAGTCCTAGGTACTATGCTAGC)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    PphsA342
  • Alt name
    Thiosulfate activated promoter
  • Alt name
    Promoter of phsA (Shal_3127)
  • Species
    Shewanella halifaxensis HAW‐EB4
  • Insert Size (bp)
    342
  • GenBank ID
    NC_010334

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    sfGFP
  • Alt name
    superfolder GFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • GenBank ID
  • Promoter PphsA342

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    AxeTxe
  • Alt name
    Toxin-antitoxin system
  • Species
    Enterococcus faecium
  • Insert Size (bp)
    1212
  • GenBank ID
    AF507977.1
  • Promoter Native

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC)

Cloning Information for Gene/Insert 6

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.23.614598 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MB 45t3 thsS(t3)R-sfGFP_mCherry was a gift from Mikhail Shapiro (Addgene plasmid # 232470 ; http://n2t.net/addgene:232470 ; RRID:Addgene_232470)

  • For your References section:

    Probiotic acoustic biosensors for noninvasive imaging of gut inflammation. Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG. Nat Commun. 2025 Aug 25;16(1):7931. doi: 10.1038/s41467-025-62569-1. 10.1038/s41467-025-62569-1 PubMed 40855083
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