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MB 98w3 ttrSR(m13)-Bxb1_P7-bARGSer
(Plasmid #232473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232473 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD33
  • Backbone manufacturer
    Beckwith Lab
  • Backbone size w/o insert (bp) 5352
  • Total vector size (bp) 27397
  • Modifications to backbone
    point mutations introduced during cloning
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    ttrS
  • Alt name
    Tetrathionate sensor kinase ttrS (Sbal195_3859)
  • Species
    Shewanella baltica OS195
  • Insert Size (bp)
    2016
  • GenBank ID
    NC_009997
  • Promoter J23109 (tttacagctagctcagtcctagggactgtgctagc)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ttrR
  • Alt name
    Tetrathionate response regulator ttrR (Sbal195_3858)
  • Species
    Shewanella baltica OS195
  • Insert Size (bp)
    621
  • GenBank ID
    NC_009997
  • Promoter Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    PttrB185-269
  • Alt name
    Minimal tetrathionate activated promoter
  • Alt name
    Truncated promoter of ttrB (Sbal195_3860)
  • Species
    Shewanella baltica OS195
  • Insert Size (bp)
    85
  • GenBank ID
    NC_009997

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    bARGSer
  • Alt name
    Bacterial acoustic reporter genes originating from Serratia sp. ATCC 39006
  • Alt name
    Modified gas vesicle gene cluster from Serratia sp. ATCC 39006
  • Species
    Serratia sp. ATCC 39006
  • Insert Size (bp)
    16692
  • Mutation
    the gene Ser39006_001280 was deleted from the cluster, and 3 point mutations were introduced during cloning (GvpG P133P, GvpW P609H, GvpZ D112Y)
  • GenBank ID
    AWXH00000000.1
  • Promoter P7 (aaaaaatttatttgctttcgcatctttttgtacctataatgtgtgga)

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    AxeTxe
  • Alt name
    Toxin-antitoxin system
  • Species
    Enterococcus faecium
  • Insert Size (bp)
    1212
  • GenBank ID
    AF507977.1
  • Promoter Native

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    Bxb1 integrase
  • Species
    Mycobacterium phage Bxb1
  • Insert Size (bp)
    1541
  • GenBank ID
    NC_002656
  • Promoter PttrB185-269
  • Tag / Fusion Protein
    • ssrA degradation tag (C terminal on insert)

Cloning Information for Gene/Insert 6

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.23.614598 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MB 98w3 ttrSR(m13)-Bxb1_P7-bARGSer was a gift from Mikhail Shapiro (Addgene plasmid # 232473 ; http://n2t.net/addgene:232473 ; RRID:Addgene_232473)

  • For your References section:

    Probiotic acoustic biosensors for noninvasive imaging of gut inflammation. Buss MT, Zhu L, Kwon JH, Tabor JJ, Shapiro MG. Nat Commun. 2025 Aug 25;16(1):7931. doi: 10.1038/s41467-025-62569-1. 10.1038/s41467-025-62569-1 PubMed 40855083
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