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pB-TR-hCAII
(Plasmid #232480)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232480 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified PB-CuO-CMV-MCS-EF1a-CymR-T2A-Puro piggyBac
  • Backbone manufacturer
    SBI -System Biosciences (original plasmid inducible PiggyBac Cumate Switch vector)
  • Backbone size w/o insert (bp) 8848
  • Total vector size (bp) 9632
  • Modifications to backbone
    the cumate operator (CuO) and repressor (CuR) in original vector were replaced with the tetracycline operator (TetO) and repressor (TetRep; TR)
  • Vector type
    Mammalian Expression ; transposon system
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human carbonic anhydrase II
  • Alt name
    CAII
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    780
  • GenBank ID
    M36532.1
  • Entrez Gene
    CA2 (a.k.a. CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282)
  • Promoter CMV-TetO
  • Tag / Fusion Protein
    • DYKDDDDK (FLAG-tag) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AGACGCCATCCACGCTGTTTTGACCTC
  • 3′ sequencing primer agggtgcgtacggccctggggacgt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-TR-hCAII was a gift from Lukas Trantirek (Addgene plasmid # 232480 ; http://n2t.net/addgene:232480 ; RRID:Addgene_232480)

  • For your References section:

    Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models. Rynes J, Istvankova E, Dzurov Krafcikova M, Luchinat E, Barbieri L, Banci L, Kamarytova K, Loja T, Fafilek B, Rico-Llanos G, Krejci P, Macurek L, Foldynova-Trantirkova S, Trantirek L. Commun Biol. 2025 Feb 7;8(1):194. doi: 10.1038/s42003-025-07607-w. 10.1038/s42003-025-07607-w PubMed 39920376
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