ubbr:GCaMP8m
(Plasmid
#232485)
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PurposeTo make fish transgenic lines that have calcium sensor expression in the whole body of the fish
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Depositing Lab
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Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 232485-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 232485-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonetol2
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namejGCaMP8m
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SpeciesD. rerio (zebrafish)
- Promoter ubbr
Cloning Information
- Cloning method Other
- 5′ sequencing primer X
- 3′ sequencing primer X
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFrom Dr.s Darius Balciunas and Justas Lazutka
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.21.586128 for bioRxiv preprint.
ubbr:GCaMP8m (ZT013)
DNA (Catalog # 232485-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 232485-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ubbr:GCaMP8m was a gift from Chie Satou (Addgene plasmid # 232485 ; http://n2t.net/addgene:232485 ; RRID:Addgene_232485) -
For your References section:
Imaging cellular activity across all organs reveals body-wide circuits. Ruetten VMS, Zheng W, Siwanowicz I, Mensh BD, Eddison M, Hu A, Chi Y, Lemire AL, Guo C, Kadobianskyi M, Renz M, Lelek-Greskovic S, He Y, Close K, Ihrke G, Dev A, Petruncio A, Wan Y, Zhang R, Fishman MC, Engert F, Judkewitz B, Rubinov M, Keller PJ, Satou C, Yu G, Tillberg PW, Sahani M, Ahrens MB. Nature. 2026 Sep 9. doi: 10.1038/s41586-026-10979-6. 10.1038/s41586-026-10979-6 PubMed 42717095
