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pBEL2913
(Plasmid #232523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232523 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YebST
  • Alt name
    LetAB
  • Species
    E. coli
  • Mutation
    LetA ΔZnR-N
  • Entrez Gene
    letA (a.k.a. b1833, ECK1832, yebS)
  • Entrez Gene
    letB (a.k.a. b1834, ECK1833, yebT)
  • Promoter araBAD
  • Tag / Fusion Protein
    • 6xHis2xQH-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.21.644421 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2913 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 232523 ; http://n2t.net/addgene:232523 ; RRID:Addgene_232523)

  • For your References section:

    LetA defines a structurally distinct transporter family involved in lipid trafficking. Santarossa CC, Li Y, Yousef S, Hasdemir HS, Rodriguez CC, Haase MB, Baek M, Coudray N, Pavek JG, Focke KN, Silverberg AL, Bautista C, Yeh J, Marty MT, Baker D, Tajkhorshid E, Ekiert DC, Bhabha G. bioRxiv 2025.03.21.644421 10.1101/2025.03.21.644421
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