pRExT2A-NT-PmSc
              
              
                (Plasmid
                
                #232548)
              
            
            
            
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            PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarlet
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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232548 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepUC57 simple
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              Vector typeBacterial Expression
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                Selectable markersPuromycin ; in Trypanosoma brucei
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberUnknown
 
Gene/Insert
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                Gene/Insert namemScarlet
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                    SpeciesSynthetic
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                  MutationNone
 
Cloning Information
- Cloning method Gene Synthesis
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRExT2A-NT-PmSc was a gift from Calvin Tiengwe (Addgene plasmid # 232548 ; http://n2t.net/addgene:232548 ; RRID:Addgene_232548) - 
                
For your References section:
A high-throughput protein tagging toolkit that retains endogenous untranslated regions for studying gene regulation in kinetoplastids. Carbajo CG, Han X, Savur B, Upadhyaya A, Taha F, Tinti M, Wheeler RJ, Yates PA, Tiengwe C. Open Biol. 2025 Feb;15(2):240334. doi: 10.1098/rsob.240334. Epub 2025 Feb 26. 10.1098/rsob.240334 PubMed 39999874