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pAGM1287_Barnase
(Plasmid #232556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232556 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAGM1287
  • Backbone manufacturer
    Sylvestre Marillonnet (Addgene plasmid # 47996)
  • Vector type
    Coding Sequence

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Barnase
  • Alt name
    Intronized Barnase
  • Species
    other

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGM1287_Barnase was a gift from Andreas Houben (Addgene plasmid # 232556 ; http://n2t.net/addgene:232556 ; RRID:Addgene_232556)

  • For your References section:

    Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction. Somasundaram S, Yaşar S, Fuchs J, Cuacos M, Claassen J, Weiss O, Kochevenko A, Lamb JC, Li T, Capdeville N, Puchta H, Houben A. Plant Communications, 30 Mar 2026: 101837. doi: 10.1016/j.xplc.2026.101837 10.1016/j.xplc.2026.101837
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