pAGM1287_NbALFA
(Plasmid
#232564)
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PurposeLevel-0 module; NbALFA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAGM1287
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Backbone manufacturerSylvestre Marillonnet (Addgene plasmid # 47996)
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Vector typeCoding Sequence
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNbALFA
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Speciesother
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGM1287_NbALFA was a gift from Andreas Houben (Addgene plasmid # 232564 ; http://n2t.net/addgene:232564 ; RRID:Addgene_232564) -
For your References section:
Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction. Somasundaram S, Yaşar S, Fuchs J, Cuacos M, Claassen J, Weiss O, Kochevenko A, Lamb JC, Li T, Capdeville N, Puchta H, Houben A. Plant Communications, 30 Mar 2026: 101837. doi: 10.1016/j.xplc.2026.101837 10.1016/j.xplc.2026.101837